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Reliability of human cortical organoid generation.
Yoon, Se-Jin; Elahi, Lubayna S; Pașca, Anca M; Marton, Rebecca M; Gordon, Aaron; Revah, Omer; Miura, Yuki; Walczak, Elisabeth M; Holdgate, Gwendolyn M; Fan, H Christina; Huguenard, John R; Geschwind, Daniel H; Pașca, Sergiu P.
Affiliation
  • Yoon SJ; Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Stanford, CA, USA.
  • Elahi LS; Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Stanford, CA, USA.
  • Pașca AM; Department of Pediatrics, Division of Neonatology, Stanford University School of Medicine, Stanford, CA, USA.
  • Marton RM; Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Stanford, CA, USA.
  • Gordon A; Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
  • Revah O; Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Stanford, CA, USA.
  • Miura Y; Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Stanford, CA, USA.
  • Walczak EM; BD Biosciences, Menlo Park, CA, USA.
  • Holdgate GM; BD Biosciences, Menlo Park, CA, USA.
  • Fan HC; BD Biosciences, Menlo Park, CA, USA.
  • Huguenard JR; Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA, USA.
  • Geschwind DH; Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
  • Pașca SP; Department of Human Genetics, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Nat Methods ; 16(1): 75-78, 2019 01.
Article in En | MEDLINE | ID: mdl-30573846
The differentiation of pluripotent stem cells in three-dimensional cultures can recapitulate key aspects of brain development, but protocols are prone to variable results. Here we differentiated multiple human pluripotent stem cell lines for over 100 d using our previously developed approach to generate brain-region-specific organoids called cortical spheroids and, using several assays, found that spheroid generation was highly reliable and consistent. We anticipate the use of this approach for large-scale differentiation experiments and disease modeling.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Organoids / Tissue Engineering Type of study: Prognostic_studies Limits: Humans Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2019 Document type: Article Affiliation country: United States Country of publication: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Organoids / Tissue Engineering Type of study: Prognostic_studies Limits: Humans Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2019 Document type: Article Affiliation country: United States Country of publication: United States