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Administration of methyl palmitate prevents non-alcoholic steatohepatitis (NASH) by induction of PPAR-α.
Zhang, Li; Li, Hui-Xia; Pan, Wu-Si; Ullah Khan, Farhan; Qian, Cheng; Qi-Li, Feng-Rong; Xu, Xiaojun.
Affiliation
  • Zhang L; State Key Laboratory of Natural Medicines, China Pharmaceutical University, 210009, Nanjing, Jiangsu, China.
  • Li HX; State Key Laboratory of Natural Medicines, China Pharmaceutical University, 210009, Nanjing, Jiangsu, China.
  • Pan WS; State Key Laboratory of Natural Medicines, China Pharmaceutical University, 210009, Nanjing, Jiangsu, China.
  • Ullah Khan F; Shanghai Jiao Tong University, School of Pharmacy, 800 Dongchuan Road, Shanghai, 200240, China.
  • Qian C; Department of Nephrology, Sir Run Run Hospital, Nanjing Medical University, Nanjing, 211166, China.
  • Qi-Li FR; State Key Laboratory of Natural Medicines, China Pharmaceutical University, 210009, Nanjing, Jiangsu, China.
  • Xu X; State Key Laboratory of Natural Medicines, China Pharmaceutical University, 210009, Nanjing, Jiangsu, China; Jiangsu Key Laboratory of Drug Discovery for Metabolic Diseases, China Pharmaceutical University, 210009, Nanjing, Jiangsu, China. Electronic address: xiaojunxu@cpu.edu.cn.
Biomed Pharmacother ; 111: 99-108, 2019 Mar.
Article in En | MEDLINE | ID: mdl-30579258
BACKGROUND AND AIMS: The lack of valid therapeutic approach that can ameliorate the manifestations of NASH is a barrier to therapeutic development. Therefore, we investigate the novel role of Methyl Palmitate (MP) in preventing NASH and the possible mechanism involved. METHODS: 50 Male C57BL/6 J mice were randomly divided into 5 groups (n = 10). The control group was fed control diet; model group was fed MCD diet; MP 1 group was fed MCD diet supplemented with MP (75 mg/kg/day); MP 2 group was fed MCD plus MP diet (150 mg/kg/day); and MP 3 group was fed MCD plus MP diet (300 mg/kg/day). Histological staining's, and commercially available kits for serum ALT and AST and hepatic contents of TG, TC, MDA, SOD, and GSH were used to assess NASH. Furthermore, relative liver protein and gene expression levels were determined by Western Blot and qPCR, respectively. RESULTS: Mice fed MCD diet developed NASH, which was markedly improved by MP in a dose-dependent manner. MP treatment improved hepatic content of TG, TC, MDA, SOD and GSH and serum levels of ALT and AST. In vivo studies showed that MP treatment activated PPARα expression, that in turns, promoted ß-oxidation protein and gene expressions, suppressed TNFα, MCP1, TGFß1 and Colla1 protein and gene expression levels, contributing to the prevention of NASH. CONCLUSIONS: Our results indicated that MP could successfully prevent NASH. This effect of MP was mediated through induction of PPARα pathway. This study provides a novel therapeutic target that plays pivotal role in the prevention of NASH.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Palmitates / PPAR alpha / Non-alcoholic Fatty Liver Disease Type of study: Etiology_studies Limits: Animals / Humans / Male Language: En Journal: Biomed Pharmacother Year: 2019 Document type: Article Affiliation country: China Country of publication: France

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Palmitates / PPAR alpha / Non-alcoholic Fatty Liver Disease Type of study: Etiology_studies Limits: Animals / Humans / Male Language: En Journal: Biomed Pharmacother Year: 2019 Document type: Article Affiliation country: China Country of publication: France