The Resolution Matrix in Tomographic Multiplexing: Optimization of Inter-Parameter Cross-Talk, Relative Quantitation, and Localization.
IEEE Trans Biomed Eng
; 66(8): 2341-2351, 2019 08.
Article
in En
| MEDLINE
| ID: mdl-30582520
ABSTRACT
OBJECTIVE:
We use a resolution matrix-based Bayesian framework to compare inversion methods for tomographic fluorescence lifetime multiplexing in a diffuse medium, such as biological tissue.METHODS:
We consider three inversion methods; an asymptotic time domain (ATD) approach, based on a multiexponential analysis of time domain data, a direct time domain (DTD) approach, which is a minimum error solution, and a cross-talk constrained time domain (CCTD) inversion, which is a solution to an optimization problem that minimizes both error and cross-talk. We compare these methods using Monte Carlo simulations and time domain fluorescence measurements with tissue-mimicking phantoms.RESULTS:
The ATD approach provides high accuracy of relative quantitation and spatial localization of two fluorophores embedded in a 18-mm thick turbid medium, with concentration ratios of up to 14.25. DTD leads to significant errors in relative quantitation and localization. CCTD provides improved quantitation accuracy over DTD, and better spatial resolution compared to ATD. We present a rigorous theoretical basis for these results and provide a complete derivation of the CCTD estimator. The Bayesian analysis also leads to a formula for rapid computation of the DTD inverse operator for large-scale tomography measurements.CONCLUSION:
The ATD and CCTD inversion methods provide significant advantages over DTD for accurately estimating multiple overlapping fluorophores.SIGNIFICANCE:
Time domain fluorescence tomography, using zero cross-talk estimators, can serve as a powerful tool for quantifying multiple fluorescently labeled biological processes. The Bayesian framework presented here can be applied to general multiparameter inverse problems for the quantitative estimation of multiple overlapping parameters.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tomography, Optical
/
Molecular Imaging
Type of study:
Health_economic_evaluation
Language:
En
Journal:
IEEE Trans Biomed Eng
Year:
2019
Document type:
Article