Gold nanoparticles of different shape for bicolor lateral flow test.
Anal Biochem
; 568: 7-13, 2019 03 01.
Article
in En
| MEDLINE
| ID: mdl-30582897
ABSTRACT
Spherical gold nanoparticles are the most commonly used marker in lateral flow assays. However, the widespread practice of using identical coloration for the test and control zones of test strips can lead to erroneous interpretations of the assay's results. We propose an immunochromatographic test strip with lines of different colors. For this purpose, gold nanoparticles of different shapes were used, namely blue nanoflowers in the test zone and red gold nanospheres in the control zone. A detailed synthesis procedure for nanoparticles and their conjugates is considered and design parameters for optimal results are described. For the first time, nanoparticles of different shapes have been combined in the test strip with indirect labeling of specific antibodies (via their interaction with labeled secondary antibodies). Using the T-2 toxin (T2T) as an example, an instrumental detection limit of 30â¯pg/ml and a working range 0.06-0.9â¯ng/mL were achieved in an analysis of water-organic corn extracts.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Reagent Strips
/
Color
/
Metal Nanoparticles
/
Gold
/
Antibodies
Limits:
Humans
Language:
En
Journal:
Anal Biochem
Year:
2019
Document type:
Article
Affiliation country:
RUSSIA