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[Simultaneous determination of insecticide in tea samples using QuEChERS-based clean up and ultra-fast liquid chromatography-tandem mass spectrometry].
Pan, Shengdong; Ye, Meijun; Chen, Xiaohong; Wang, Li; Jin, Micong.
Affiliation
  • Pan S; Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China.
  • Ye M; Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China.
  • Chen X; Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China.
  • Wang L; Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China.
  • Jin M; Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China.
Wei Sheng Yan Jiu ; 47(5): 809-814, 2018 Sep.
Article in Zh | MEDLINE | ID: mdl-30593311
OBJECTIVE: To develop a method for determination of imidacloprid, acetamiprid, chlorobenzamide and indoxacarb in tea samples using Qu ECh ERS-based pretreatment method and ultra-fast liquid chromatography-tandem mass spectrometry( UFLC-MS/MS). METHODS: Tea samples were firstly extracted by acetonitrile-water solution( 4∶ 1, V/V) by vortex and ultrasound, and then 1 g Na Cl and 4 g Mg SO4 were added into the mixture, following by vortex and centrifugation at 8500 r/min for 5 min. Finally the supernatant was purified by Mg SO4 and PSA power, and then the chromatographic separation process was performed on a Waters ACQUITY UPLC BEH C18 column( 2. 1 mm × 100 mm, 1. 7 µm) with a linear gradient elution procedure ofacetonitrile and 0. 1%( V/V) formic acid-5 mmol/L ammonium acetate in water as elution solvent. The multiple reaction monitoring( MRM) in positive mode was used for quantification by internal standard method. RESULTS: The four insecticides including imidacloprid, acetamiprid, chlorobenzamide, and indoxacarb showed good linearity in the range of 0. 20-50. 0 µg/L with coefficients( r) higher than 0. 9998. The limits of detection( LODs) varied from 0. 1 µg/kg to 0. 3 µg/kg. The recoveries of spiked tea samples in the range of 88. 4%-98. 8% at the three concentrations of 1. 0 µg/kg, 40. 0µg/kg and 80. 0 µg/kg, while the relative standard deviations( RSD) were all less than10%. CONCLUSION: The proposed method is simple, fast, sensitive and accuracy, and can be used for qualitative and quantitative analysis of imidacloprid, acetamiprid, chlorobenzamide, andindoxacarb in tea samples.
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Collection: 01-internacional Database: MEDLINE Main subject: Tea / Food Contamination / Insecticides Type of study: Qualitative_research Language: Zh Journal: Wei Sheng Yan Jiu Journal subject: SAUDE PUBLICA Year: 2018 Document type: Article Affiliation country: China Country of publication: China
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Collection: 01-internacional Database: MEDLINE Main subject: Tea / Food Contamination / Insecticides Type of study: Qualitative_research Language: Zh Journal: Wei Sheng Yan Jiu Journal subject: SAUDE PUBLICA Year: 2018 Document type: Article Affiliation country: China Country of publication: China