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Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults.
Vlahos, Katerina; Sourris, Koula; Mayberry, Robyn; McDonald, Penelope; Bruveris, Freya F; Schiesser, Jacqueline V; Bozaoglu, Kiymet; Lockhart, Paul J; Stanley, Edouard G; Elefanty, Andrew G.
Affiliation
  • Vlahos K; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia. Electronic address: katerina.vlahos@mcri.edu.au.
  • Sourris K; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia.
  • Mayberry R; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia.
  • McDonald P; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia.
  • Bruveris FF; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, Australia.
  • Schiesser JV; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, Australia.
  • Bozaoglu K; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, Australia; Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, V
  • Lockhart PJ; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, Australia; Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, V
  • Stanley EG; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, Australia; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3800, Australi
  • Elefanty AG; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, Australia; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3800, Australi
Stem Cell Res ; 34: 101380, 2019 01.
Article in En | MEDLINE | ID: mdl-30605840
We describe the generation and characterization of 5 human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. The iPSC lines exhibited a normal karyotype, expressed pluripotency markers and differentiated into cells representative of the three embryonic germ layers. These iPSC lines can be used as controls in studying disease mechanisms.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Leukocytes, Mononuclear / Cell Culture Techniques / Induced Pluripotent Stem Cells Limits: Adult / Female / Humans / Male / Middle aged Language: En Journal: Stem Cell Res Year: 2019 Document type: Article Country of publication: United kingdom
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Leukocytes, Mononuclear / Cell Culture Techniques / Induced Pluripotent Stem Cells Limits: Adult / Female / Humans / Male / Middle aged Language: En Journal: Stem Cell Res Year: 2019 Document type: Article Country of publication: United kingdom