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A reverse-transcription/RNase H based protocol for depletion of mosquito ribosomal RNA facilitates viral intrahost evolution analysis, transcriptomics and pathogen discovery.
Fauver, Joseph R; Akter, Shamima; Morales, Aldo Ivan Ortega; Black, William C; Rodriguez, Americo D; Stenglein, Mark D; Ebel, Gregory D; Weger-Lucarelli, James.
Affiliation
  • Fauver JR; Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
  • Akter S; Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic Institute and State University, 360 W Campus Drive, Blacksburg, VA, USA.
  • Morales AIO; Departamento de Parasitología, Universidad Autónoma Agraria Antonio Narro, Torreón, Coahuila, Mexico.
  • Black WC; Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
  • Rodriguez AD; Centro Regional de Investigación en Salud Publica, Instituto Nacional de Salud Pública, Tapachula, Chiapas, Mexico.
  • Stenglein MD; Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
  • Ebel GD; Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA. Electronic address: Gregory.Ebel@colostate.edu.
  • Weger-Lucarelli J; Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA. Electronic address: weger@vt.edu.
Virology ; 528: 181-197, 2019 02.
Article in En | MEDLINE | ID: mdl-30616207
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: West Nile virus / RNA, Ribosomal / Ribonuclease H / Reverse Transcription / Culicidae Limits: Animals Language: En Journal: Virology Year: 2019 Document type: Article Affiliation country: United States Country of publication: United States
Fulltext
Add to My VHL
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: West Nile virus / RNA, Ribosomal / Ribonuclease H / Reverse Transcription / Culicidae Limits: Animals Language: En Journal: Virology Year: 2019 Document type: Article Affiliation country: United States Country of publication: United States