Stabilizing bromelain for therapeutic applications by adsorption immobilization on spores of probiotic Bacillus.
Int J Biol Macromol
; 127: 406-414, 2019 Apr 15.
Article
in En
| MEDLINE
| ID: mdl-30654039
ABSTRACT
Bromelain, a protease from pineapple plant can be applied as oral drug for the treatment of inflammation and certain diseases. Unlike most conventional supports, immobilization on edible support will make the enzyme suitable for therapeutic use. In this study, spores of probiotic Bacillus sp was used for the adsorption of bromelain. Effect of pH, temperature and enzyme concentration on bromelain immobilization was studied, followed by characterization of the enzymes. Maximum bromelain coupling (%) (50.607⯱â¯4.194) was obtained when immobilization was carried out at pHâ¯6.0, 24⯰C for 150â¯min. The immobilized enzyme showed optimum activity at pHâ¯8 and 80⯰C, while the free enzyme had 6 and 60⯰C as its optimum pH and temperature, respectively. Bromelain Vmax increased after immobilization while Km decreased. Activation energy, Ea was 26.513â¯kJ/mol and 20.942â¯kJ/mol for the free and immobilized enzymes, respectively. The immobilized bromelain also showed significantly greater storage and thermal stability than the free bromelain. At 80⯰C, the free bromelain lost all its activity after 50â¯min while the immobilized enzyme lost only 46.89% activity. Bromelain was successfully immobilized on Bacillus spores with improved catalytic and non-catalytic properties and this holds great potential for its growing therapeutic applications.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Proteins
/
Spores, Bacterial
/
Bacillus
/
Bromelains
/
Probiotics
/
Ananas
/
Enzymes, Immobilized
Language:
En
Journal:
Int J Biol Macromol
Year:
2019
Document type:
Article