Cortical column and whole-brain imaging with molecular contrast and nanoscale resolution.
Science
; 363(6424)2019 01 18.
Article
in En
| MEDLINE
| ID: mdl-30655415
ABSTRACT
Optical and electron microscopy have made tremendous inroads toward understanding the complexity of the brain. However, optical microscopy offers insufficient resolution to reveal subcellular details, and electron microscopy lacks the throughput and molecular contrast to visualize specific molecular constituents over millimeter-scale or larger dimensions. We combined expansion microscopy and lattice light-sheet microscopy to image the nanoscale spatial relationships between proteins across the thickness of the mouse cortex or the entire Drosophila brain. These included synaptic proteins at dendritic spines, myelination along axons, and presynaptic densities at dopaminergic neurons in every fly brain region. The technology should enable statistically rich, large-scale studies of neural development, sexual dimorphism, degree of stereotypy, and structural correlations to behavior or neural activity, all with molecular contrast.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Brain
/
Nanotechnology
/
Neuroimaging
/
Optical Imaging
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
En
Journal:
Science
Year:
2019
Document type:
Article
Affiliation country:
United States