Development of a GC-MS/SIM method for the determination of phytosteryl esters.
Food Chem
; 281: 236-241, 2019 May 30.
Article
in En
| MEDLINE
| ID: mdl-30658753
ABSTRACT
A gas chromatography-mass spectrometry (GC-MS) method with selected ion monitoring (SIM) was developed and validated for identification and quantitative analysis of three phytosteryl esters, i.e., campesteryl oletate, stigmasteryl oletate and ß-sitosteryl oletate. The method is simple and efficient and achieved good separation of the three phytosteryl esters in 10â¯min without saponification and liquid-liquid extraction. A calibration curve for the three phytosteryl esters had a correlation coefficient (R2) better than 0.993. Detection limits were 0.42â¯mg/mL for campesteryl oletate, 0.32â¯mg/mL for stigmasteryl oletate and 0.80â¯mg/mL for ß-sitosteryl oletate. The relative standard deviations (RSD) were within 5.47% for precision and stability for three edible oil samples. Recoveries were from 89.85% to 97.65% for each of the phytosteryl esters. These results suggest that the method can be used to identify and quantify the phytosteryl esters in oil samples.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Esters
/
Gas Chromatography-Mass Spectrometry
Type of study:
Diagnostic_studies
/
Prognostic_studies
Language:
En
Journal:
Food Chem
Year:
2019
Document type:
Article
Affiliation country:
China