Capillary electrophoresis integrated immobilized enzyme reactor for kinetic and inhibition assays of ß-secretase as the Alzheimer's disease drug target.

Schejbal, Jan; Sefraná, Sárka; Remínek, Roman; Glatz, Zdenek

Schejbal, Jan; Sefraná, Sárka; Remínek, Roman; Glatz, Zdenek.

Affiliation

Schejbal J; Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.
Sefraná S; Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.
Remínek R; Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.
Glatz Z; Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.

J Sep Sci ; 42(5): 1067-1076, 2019 Mar.

Article in En | MEDLINE | ID: mdl-30663871

Subject(s)

Alzheimer Disease/drug therapy; Amyloid Precursor Protein Secretases/antagonists & inhibitors; Bioreactors; Enzyme Inhibitors/pharmacology; Peptides/pharmacology; Alzheimer Disease/metabolism; Amyloid Precursor Protein Secretases/chemistry; Amyloid Precursor Protein Secretases/metabolism; Electrophoresis, Capillary; Enzyme Inhibitors/chemistry; Enzymes, Immobilized/antagonists & inhibitors; Enzymes, Immobilized/chemistry; Enzymes, Immobilized/metabolism; HEK293 Cells; Humans; Kinetics; Peptides/chemistry

Key words

capillary electrophoresis; immobilized enzyme reactor; inhibition; magnetic particles; secretase

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptides / Bioreactors / Enzyme Inhibitors / Amyloid Precursor Protein Secretases / Alzheimer Disease Limits: Humans Language: En Journal: J Sep Sci Year: 2019 Document type: Article Affiliation country: Czech Republic Country of publication: Germany

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