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A genetically encoded near-infrared fluorescent calcium ion indicator.
Qian, Yong; Piatkevich, Kiryl D; Mc Larney, Benedict; Abdelfattah, Ahmed S; Mehta, Sohum; Murdock, Mitchell H; Gottschalk, Sven; Molina, Rosana S; Zhang, Wei; Chen, Yingche; Wu, Jiahui; Drobizhev, Mikhail; Hughes, Thomas E; Zhang, Jin; Schreiter, Eric R; Shoham, Shy; Razansky, Daniel; Boyden, Edward S; Campbell, Robert E.
Affiliation
  • Qian Y; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Piatkevich KD; Media Lab and McGovern Institute for Brain Research, MIT, Cambridge, MA, USA.
  • Mc Larney B; Institute for Biological and Medical Imaging, Helmholtz Center Munich, Neuherberg, Germany.
  • Abdelfattah AS; Faculty of Medicine, Technical University of Munich, Munich, Germany.
  • Mehta S; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
  • Murdock MH; Department of Pharmacology, University of California San Diego, La Jolla, CA, USA.
  • Gottschalk S; Media Lab and McGovern Institute for Brain Research, MIT, Cambridge, MA, USA.
  • Molina RS; Institute for Biological and Medical Imaging, Helmholtz Center Munich, Neuherberg, Germany.
  • Zhang W; Department of Cell Biology and Neuroscience, Montana State University, Bozeman, MT, USA.
  • Chen Y; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Wu J; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Drobizhev M; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Hughes TE; Department of Cell Biology and Neuroscience, Montana State University, Bozeman, MT, USA.
  • Zhang J; Department of Cell Biology and Neuroscience, Montana State University, Bozeman, MT, USA.
  • Schreiter ER; Department of Pharmacology, University of California San Diego, La Jolla, CA, USA.
  • Shoham S; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
  • Razansky D; Departments of Ophthalmology and of Neuroscience and Physiology, New York University Langone Health, New York City, NY, USA.
  • Boyden ES; Institute for Biological and Medical Imaging, Helmholtz Center Munich, Neuherberg, Germany.
  • Campbell RE; Faculty of Medicine, Technical University of Munich, Munich, Germany.
Nat Methods ; 16(2): 171-174, 2019 02.
Article in En | MEDLINE | ID: mdl-30664778
ABSTRACT
We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca2+) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca2+ transients in cultured mammalian cells and brain tissue with sensitivity comparable to that of currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca2+ imaging in combination with other optogenetic indicators and actuators.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Calcium / Spectroscopy, Near-Infrared / Fluorescent Dyes / Microscopy, Fluorescence Limits: Animals / Female / Humans / Male Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2019 Document type: Article Affiliation country: Canada
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Calcium / Spectroscopy, Near-Infrared / Fluorescent Dyes / Microscopy, Fluorescence Limits: Animals / Female / Humans / Male Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2019 Document type: Article Affiliation country: Canada