Human peripheral blood monocytes secrete a unique form of PDGF.
Oncogene Res
; 3(4): 333-42, 1988.
Article
in En
| MEDLINE
| ID: mdl-3067188
ABSTRACT
PDGF isolated from platelets and forms of PDGF produced by cells transformed with the v-sis gene (PDGF B chain) or U-20S osteosarcoma cells which express the PDGF A chain gene are known to be processed as disulfide-linked dimers of approximately 30 kd. Western blot analysis with anti human PDGF antibody of the PDGF-like factor synthesized and secreted by human blood monocytes (MDGF) on SDS gels indicates that it lacks interchain disulfide bridges and behaves as a 16-kd monomer under nonreducing conditions. Additionally, MDGF exhibits different sensitivities to either formic acid or CNBr cleavage compared to PDGF A or B chain molecules indicating it may have a different primary structure. These data suggest that MDGF represents a unique form of PDGF which lacks interchain disulfide bridges and may represent a new member of the PDGF family of growth factors.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Platelet-Derived Growth Factor
/
Monocytes
Limits:
Humans
Language:
En
Journal:
Oncogene Res
Year:
1988
Document type:
Article