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Analysis of pectin from Panax ginseng flower buds and their binding activities to galectin-3.
Cui, Liangnan; Wang, Jiayi; Huang, Rui; Tan, Ya; Zhang, Fan; Zhou, Yifa; Sun, Lin.
Affiliation
  • Cui L; Jilin Province Key Laboratory on Chemistry and Biology of Natural Drugs in Changbai Mountain, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China. Electronic address: cuiln802@nenu.edu.cn.
  • Wang J; Jilin Province Key Laboratory on Chemistry and Biology of Natural Drugs in Changbai Mountain, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China. Electronic address: wangjy753@nenu.edu.cn.
  • Huang R; Jilin Province Key Laboratory on Chemistry and Biology of Natural Drugs in Changbai Mountain, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China. Electronic address: huangr032@nenu.edu.cn.
  • Tan Y; Jilin Province Key Laboratory on Chemistry and Biology of Natural Drugs in Changbai Mountain, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China. Electronic address: tany762@nenu.edu.cn.
  • Zhang F; Jilin Province Key Laboratory on Chemistry and Biology of Natural Drugs in Changbai Mountain, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China. Electronic address: zhangf508@nenu.edu.cn.
  • Zhou Y; Jilin Province Key Laboratory on Chemistry and Biology of Natural Drugs in Changbai Mountain, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China. Electronic address: zhouyf383@nenu.edu.cn.
  • Sun L; Jilin Province Key Laboratory on Chemistry and Biology of Natural Drugs in Changbai Mountain, School of Life Sciences, Northeast Normal University, Changchun 130024, PR China. Electronic address: sunl925@nenu.edu.cn.
Int J Biol Macromol ; 128: 459-467, 2019 May 01.
Article in En | MEDLINE | ID: mdl-30703424
ABSTRACT
Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies. Monosaccharide composition, enzymatic hydrolysis and 13C nuclear magnetic resonance (NMR) spectra analysis were combined to characterize their structural features. Furthermore, the interactions between these polysaccharides and galectin-3 were evaluated by biolayer interferometry assay. The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, ß-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 µM, 0.71 µM and 0.24 µM, respectively. WGFPA-1b, WGFPA-2b and WGFPA-3b were homogalacturonan (HG) type pectin covalently linked with different ratios of rhamnogalacturonan II (RG-II) domains, showing weaker or no interactions with galectin-3. This study provides useful structural information for further investigation on the structure-activity relationship of ginseng flower buds pectin.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pectins / Flowers / Galectin 3 / Panax Language: En Journal: Int J Biol Macromol Year: 2019 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pectins / Flowers / Galectin 3 / Panax Language: En Journal: Int J Biol Macromol Year: 2019 Document type: Article