Enzyme self-assembly on naked iron oxide nanoparticles for aminoaldehyde biosensing.
Amino Acids
; 51(4): 679-690, 2019 Apr.
Article
in En
| MEDLINE
| ID: mdl-30725223
ABSTRACT
The preservation of enzymatic activity is a fundamental requirement for exploiting hybrid nano-bio-conjugates, and the control over protein-nanoparticle interactions, leading to stable and catalytically active hybrids, represents the key for designing new biosensing platforms. In this scenario, surface active maghemite nanoparticles (SAMNs) represent a new class of naked magnetic nanoparticles, displaying peculiar electrocatalytic features and the ability to selectively bind proteins. Recombinant aminoaldehyde dehydrogenase from tomato (SlAMADH1) was used as a model protein, and successfully immobilized by self-assembly on the surface of naked SAMNs, where its enzymatic activity resulted preserved for more than 6 months. The hybrid nanomaterial (SAMN@SlAMADH1) was characterized by UV-Vis spectroscopy, mass spectrometry, and TEM microscopy, and applied for the development of a biosensor for the determination of aminoaldehydes in alcoholic beverages. Measurements were carried out in a low volume electrochemical flow cell comprising a SAMN modified carbon paste electrode for the coulometric determination of the NADH produced during the enzymatic catalysis. The present findings, besides representing the first example of an electrochemical biosensor for aminoaldehydes in an alcoholic matrix, open the door to the use of immobilized enzymes on naked metal oxides nanomaterials for biosensing.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Propylamines
/
Ferric Compounds
/
Biosensing Techniques
/
Solanum lycopersicum
/
Aldehyde Dehydrogenase
/
Aldehydes
/
Enzymes, Immobilized
/
Metal Nanoparticles
Type of study:
Prognostic_studies
Language:
En
Journal:
Amino Acids
Journal subject:
BIOQUIMICA
Year:
2019
Document type:
Article
Affiliation country:
Italy