CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding.
Commun Biol
; 2: 46, 2019.
Article
in En
| MEDLINE
| ID: mdl-30729184
ABSTRACT
Presence of the integrated endogenous banana streak virus (eBSV) in the B genome of plantain (AAB) is a major challenge for breeding and dissemination of hybrids. As the eBSV activates into infectious viral particles under stress, the progenitor Musa balbisiana and its derivants, having at least one B genome, cannot be used as parents for crop improvement. Here, we report a strategy to inactivate the eBSV by editing the virus sequences. The regenerated genome-edited events of Gonja Manjaya showed mutations in the targeted sites with the potential to prevent proper transcription or/and translational into functional viral proteins. Seventy-five percent of the edited events remained asymptomatic in comparison to the non-edited control plants under water stress conditions, confirming inactivation of eBSV into infectious viral particles. This study paves the way for the improvement of B genome germplasm and its use in breeding programs to produce hybrids that can be globally disseminated.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Genome, Viral
/
Genome, Plant
/
Badnavirus
/
Musa
/
CRISPR-Cas Systems
/
Plant Breeding
/
Gene Editing
Language:
En
Journal:
Commun Biol
Year:
2019
Document type:
Article
Affiliation country:
Kenya