Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons.
J Vis Exp
; (143)2019 01 18.
Article
in En
| MEDLINE
| ID: mdl-30735185
ABSTRACT
Recent whole-cell patch-clamp studies from substantia gelatinosa (SG) neurons have provided a large body of information about the spinal mechanisms underlying sensory transmission, nociceptive regulation, and chronic pain or itch development. Implementations of electrophysiological recordings together with morphological studies based on the utility of acute spinal cord slices have further improved our understanding of neuronal properties and the composition of local circuitry in SG. Here, we present a detailed and practical guide for the preparation of spinal cord slices and show representative whole-cell recording and morphological results. This protocol permits ideal neuronal preservation and can mimic in vivo conditions to a certain extent. In summary, the ability to obtain an in vitro preparation of spinal cord slices enables stable current- and voltage-clamp recordings and could thus facilitate detailed investigations into the intrinsic membrane properties, local circuitry and neuronal structure using diverse experimental approaches.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Substantia Gelatinosa
/
Patch-Clamp Techniques
/
Neurons
Type of study:
Guideline
Limits:
Animals
Language:
En
Journal:
J Vis Exp
Year:
2019
Document type:
Article