Transgenic yellow lupin (Lupinus luteus).
Plant Cell Rep
; 19(6): 634-637, 2000 May.
Article
in En
| MEDLINE
| ID: mdl-30754829
Transgenic yellow lupin (Lupinus luteus L.) plants have been generated by meristem co-cultivation with Agrobacterium tumefaciens. The binary plasmid pPZBNIa contains the bar gene under the control of a CaMV 35 S promoter. The transformation method involves inoculation of embryonic axis explants with A. tumefaciens, flooding the meristem with glufosinate, and initial culture on non-selective medium. Shoots were transferred to culture medium containing 20 mg/l glufosinate. Following subculture, shoots were grafted onto non-transgenic narrow-leafed lupin (L. angustifolius L.) seedling rootstocks, or rooted in vitro. The overall transformation efficiency, as determined at the T1 generation, was 0.05%-0.75%. The transgenic nature of plants grown to the T6 generation was confirmed by phosphinothricin acetyl transferase, PCR and Southern analyses.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Plant Cell Rep
Journal subject:
BOTANICA
Year:
2000
Document type:
Article
Country of publication:
Germany