Production of Extracellular Matrix Fibers via Sacrificial Hollow Fiber Membrane Cell Culture.
J Vis Exp
; (144)2019 02 02.
Article
in En
| MEDLINE
| ID: mdl-30774136
ABSTRACT
Engineered scaffolds derived from extracellular matrix (ECM) have driven significant interest in medicine for their potential in expediting wound closure and healing. Extraction of extracellular matrix from fibrogenic cell cultures in vitro has potential for generation of ECM from human- and potentially patient-specific cell lines, minimizing the presence of xenogeneic epitopes which has hindered the clinical success of some existing ECM products. A significant challenge in in vitro production of ECM suitable for implantation is that ECM production by cell culture is typically of relatively low yield. In this work, protocols are described for the production of ECM by cells cultured within sacrificial hollow fiber membrane scaffolds. Hollow fiber membranes are cultured with fibroblast cell lines in a conventional cell medium and dissolved after cell culture to yield continuous threads of ECM. The resulting ECM fibers produced by this method can be decellularized and lyophilized, rendering it suitable for storage and implantation.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tissue Engineering
/
Extracellular Matrix
Type of study:
Guideline
Limits:
Humans
Language:
En
Journal:
J Vis Exp
Year:
2019
Document type:
Article