Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples.
Braz J Med Biol Res
; 52(2): e8194, 2019 Feb 14.
Article
in En
| MEDLINE
| ID: mdl-30785480
ABSTRACT
Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bone marrow (BM, n=352) and peripheral blood (PB, n=69) samples were analyzed from acute myeloid leukemia (n=113), myelodysplastic syndromes (n=73), myelodysplastic syndromes/myeloproliferative neoplasms (n=17), myeloproliferative neoplasms (n=137), and other with conclusive diagnosis (n=6), and reactive disorders/no conclusive diagnosis (n=75). The rate of unsuccessful karyotyping was 18.5% and was associated with the use of PB and a low number of nucleated cells (≤7×103/µL) in the sample. High and low cellularity in BM and high and low cellularity in PB samples showed no metaphases in 3.9, 39.7, 41.9, and 84.6% of cases, respectively. Collecting a good BM sample is the key for the success of karyotyping in MN and avoids the use of expensive molecular techniques.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Specimen Handling
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Myelodysplastic Syndromes
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Bone Marrow Cells
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Leukemia, Myeloid
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Karyotyping
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Myeloproliferative Disorders
Type of study:
Diagnostic_studies
/
Risk_factors_studies
Limits:
Adolescent
/
Adult
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Aged
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Aged80
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Female
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Humans
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Male
/
Middle aged
Language:
En
Journal:
Braz J Med Biol Res
Year:
2019
Document type:
Article
Affiliation country:
Brazil