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Cochlear outer hair cell horizontal top connectors mediate mature stereocilia bundle mechanics.
Cartagena-Rivera, Alexander X; Le Gal, Sébastien; Richards, Kerianne; Verpy, Elisabeth; Chadwick, Richard S.
Affiliation
  • Cartagena-Rivera AX; Section on Auditory Mechanics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA.
  • Le Gal S; Unité de Génétique et Physiologie de l'Audition, Institut Pasteur, 75015 Paris, France.
  • Richards K; UMRS 1120, Institut National de la Santé et de la Recherche Médicale (INSERM), 75015 Paris, France.
  • Verpy E; Sorbonne Universités, Université Pierre et Marie Curie Paris 06, Complexité du Vivant, 75005 Paris, France.
  • Chadwick RS; Genomics and Computational Biology Core, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA.
Sci Adv ; 5(2): eaat9934, 2019 02.
Article in En | MEDLINE | ID: mdl-30801007
ABSTRACT
Outer hair cell (OHC) stereocilia bundle deflection opens mechanoelectrical transduction channels at the tips of the stereocilia from the middle and short rows, while bundle cohesion is maintained owing to the presence of horizontal top connectors. Here, we used a quantitative noncontact atomic force microscopy method to investigate stereocilia bundle stiffness and damping, when stimulated at acoustic frequencies and nanometer distances from the bundle. Stereocilia bundle mechanics were determined in stereocilin-deficient mice lacking top connectors and with detached tectorial membrane (Strc -/-/Tecta -/- double knockout) and heterozygous littermate controls (Strc +/-/Tecta -/-). A substantial decrease in bundle stiffness and damping by ~60 and ~74% on postnatal days P13 to P15 was observed when top connectors were absent. Additionally, we followed bundle mechanics during OHC top connectors development between P9 and P15 and quantified the observed increase in OHC bundle stiffness and damping in Strc +/-/Tecta -/- mice while no significant change was detected in Strc -/-/Tecta -/- animals.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Tectorial Membrane / Hair Cells, Auditory, Outer / Intercellular Signaling Peptides and Proteins / Stereocilia Limits: Animals Language: En Journal: Sci Adv Year: 2019 Document type: Article Affiliation country: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Tectorial Membrane / Hair Cells, Auditory, Outer / Intercellular Signaling Peptides and Proteins / Stereocilia Limits: Animals Language: En Journal: Sci Adv Year: 2019 Document type: Article Affiliation country: United States