Enhanced Production of δ-Guaiene, a Bicyclic Sesquiterpene Accumulated in Agarwood, by Coexpression of δ-Guaiene Synthase and Farnesyl Diphosphate Synthase Genes in Escherichia coli.
Nat Prod Commun
; 11(9): 1221-1224, 2016 Sep.
Article
in En
| MEDLINE
| ID: mdl-30807004
ABSTRACT
Two genes involved in δ-guaiene biosynthesis in Aquilaria microcarpa, δ-guaiene synthase (GS) and famesyl diphosphate synthase (FPS), were overexpressed in Escherichia coli cells. Immunoblot analysis revealed that the concentration of GS-translated protein was rather low in the cells transformed by solely GS while appreciable accumulation of the recombinant protein was observed when GS was coexpressed with FPS. GS-transformed cells liberated only a trace amount of δ-guaiene (0.004 µg/mL culture), however, the concentration of the compound elevated to 0.08 pg/mL culture in the cells transformed by GS plus FPS. δ-Guaiene biosynthesis was markedly activated when E. coli cells coexpressing GS and FPS were incubated in enriched Terrific broth, and the content of the compound increased to approximately 0.6 µg/mL culture. These results suggest that coexpression of FPS and GS in E. coli is required for efficient δ- guaiene production in the bacterial cells, and the sesquiterpene-producing activity of the transformant is appreciably enhanced in the nutrients-enriched medium.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carbon-Carbon Lyases
/
Thymelaeaceae
/
Sesquiterpenes, Guaiane
/
Geranyltranstransferase
Language:
En
Journal:
Nat Prod Commun
Journal subject:
BOTANICA
/
FARMACOLOGIA
/
QUIMICA
Year:
2016
Document type:
Article