Identification of a novel cluster of PCV2 isolates from Meghalaya, India indicates possible recombination along with changes in capsid protein.
Infect Genet Evol
; 71: 7-15, 2019 07.
Article
in En
| MEDLINE
| ID: mdl-30807843
ABSTRACT
Documentation of the emergence of Porcine circovirus 2 (PCV2) infection and economic losses incurred due to high mortality has been reported worldwide. The prevalence and genetic diversity of the virus has been reported in Northeast India including the possible chances of Classical swine fever virus (CSFV) vaccine failure in pig population in this region resulting in major disease outbreak. Irrespective of the genetic variability, the emergence of a novel cluster (based on the ORF2 phylogeny) was reported last year. The present study describes a state-wide (Meghalaya, India) molecular epidemiological investigation of PCV2 strains in pig population by amplification, sequencing and undertaking phylogenetic analyses. The results indicate the identification of a novel cluster of PCV2 originating from the inter-genotypic recombination between PCV2c and PCV2d. Multiple sequence alignment of amino acids indicates possible substitution in the A, B and C domains of the capsid protein. Molecular structural modelling of the capsid protein of PCV2 indicated possible motif variations in the secondary structure including presence of a tunnel, encountered at the interface region on each chain facilitating in transportation of molecules and acting as an active site for attachment and penetration. The baseline data strengthens the existing control programme of PCV2 and is possibly helpful in the planning of active surveillance strategy in this region.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Swine Diseases
/
Circovirus
/
Circoviridae Infections
/
Capsid Proteins
Type of study:
Diagnostic_studies
/
Risk_factors_studies
Limits:
Animals
Country/Region as subject:
Asia
Language:
En
Journal:
Infect Genet Evol
Journal subject:
BIOLOGIA
/
DOENCAS TRANSMISSIVEIS
/
GENETICA
Year:
2019
Document type:
Article
Affiliation country:
India