Aberrant natural killer (NK) cell activation and dysfunction among ART-treated HIV-infected adults in an African cohort.
Clin Immunol
; 201: 55-60, 2019 04.
Article
in En
| MEDLINE
| ID: mdl-30817998
ABSTRACT
BACKGROUND:
We examined NK cell phenotypes and functions after seven years of ART and undetectable viral loads (<50â¯copies/ml) with restored CD4 T-cell counts (≥500â¯cells/µl) and age-matched healthy-HIV-uninfected individuals from the same community.METHODS:
Using flow-cytometry, NK cell phenotypes were described using lineage markers (CD56+/-CD16+/-). NK cell activation was determined by expression of activation receptors (NKG2D, NKp44 and NKp46) and activation marker CD69. NK cell function was determined by CD107a, granzyme-b, and IFN-gamma production.RESULTS:
CD56 dim and CD56 bright NK cells were lower among ART-treated-HIV-infected than among age-matched-HIV-negative individuals; pâ¯=â¯0.0016 and pâ¯=â¯0.05 respectively. Production of CD107a (Pâ¯=â¯0.004) and Granzyme-B (Pâ¯=â¯0.005) was lower among ART-treated-HIV-infected relative to the healthy-HIV-uninfected individuals. NKG2D and NKp46 were lower, while CD69 expression was higher among ART-treated-HIV-infected than healthy-HIV-uninfected individuals.CONCLUSION:
NK cell activation and dysfunction persisted despite seven years of suppressive ART with "normalization" of peripheral CD4 counts.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Killer Cells, Natural
/
HIV Infections
/
Anti-Retroviral Agents
Type of study:
Risk_factors_studies
Limits:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Clin Immunol
Journal subject:
ALERGIA E IMUNOLOGIA
Year:
2019
Document type:
Article
Affiliation country:
Uganda