Dione-thiophene conjugate inhibits proliferation and metastasis of nasopharyngeal carcinoma cells through calcium binding protein-P down-regulation.
Eur J Med Chem
; 168: 199-206, 2019 Apr 15.
Article
in En
| MEDLINE
| ID: mdl-30822709
ABSTRACT
In the present study a series of dione-thiophenol conjugates was prepared and evaluated against nasopharyngeal carcinoma (NPC) cells. MTT assay showed that compound 4a reduced proliferation of C666-1 and CNE-1â¯cells to 26 and 24%, respectively at 10⯵mol/l concentration. Flow cytometry revealed that increasing the concentration of compound 4a from 2 to 10⯵mol/l increased the proportion of early apoptotic C666-1â¯cells from 2.76 to 69.43%. A significant (Pâ¯<â¯0.001) decrease in the expression of S100P was caused by compound 4a. In compound 4a treated C666-1â¯cells the expression of RAGE, EGFR, CD44, MMP2 and MMP9 was markedly decreased. In summary, compound 4a inhibits nasopharyngeal cancer cell proliferation and induces apoptosis through down-regulation of S100P. Moreover, compound 4a also decreases MMP-2, MMP-9, EGFR, CD44 and RAGE expression in nasopharyngeal cancer cells. Thus, compound 4a can be investigated further as a drug candidate for the treatment of nasopharyngeal cancer.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Thiophenes
/
Calcium-Binding Proteins
/
Nasopharyngeal Neoplasms
/
Cycloheptanes
/
Nasopharyngeal Carcinoma
/
Antineoplastic Agents
Limits:
Humans
Language:
En
Journal:
Eur J Med Chem
Year:
2019
Document type:
Article
Affiliation country:
China