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Matrigel Plug Assay for In Vivo Evaluation of Angiogenesis.
Kastana, Pinelopi; Zahra, Fatema Tuz; Ntenekou, Despoina; Katraki-Pavlou, Stamatiki; Beis, Dimitris; Lionakis, Michail S; Mikelis, Constantinos M; Papadimitriou, Evangelia.
Affiliation
  • Kastana P; Laboratory of Molecular Pharmacology, Department of Pharmacy, School of Health Sciences, University of Patras, Patras, Greece.
  • Zahra FT; Department of Pharmaceutical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX, USA.
  • Ntenekou D; Laboratory of Molecular Pharmacology, Department of Pharmacy, School of Health Sciences, University of Patras, Patras, Greece.
  • Katraki-Pavlou S; Laboratory of Molecular Pharmacology, Department of Pharmacy, School of Health Sciences, University of Patras, Patras, Greece.
  • Beis D; Biomedical Research Foundation Academy of Athens, Athens, Greece.
  • Lionakis MS; Biomedical Research Foundation Academy of Athens, Athens, Greece.
  • Mikelis CM; Fungal Pathogenesis Section, Laboratory of Clinical Immunology and Microbiology (LCIM), National Institute of Allergy & Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD, USA.
  • Papadimitriou E; Department of Pharmaceutical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX, USA.
Methods Mol Biol ; 1952: 219-232, 2019.
Article in En | MEDLINE | ID: mdl-30825178
Matrigel is extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma in C57BL/6 mice, a tumor rich in extracellular matrix (ECM) proteins. It consists mainly of laminin (approximately 60%), collagen IV (approximately 30%), and nidogen-1/entactin (approximately 8%), while it also contains heparan sulfate proteoglycans, such as perlecan, other ECM proteins, as well as growth factors bound to the ECM. Matrigel mimics the physiological cell matrix and is the most commonly used matrix substrate to study in vitro and in vivo angiogenesis. Here, we describe the in vivo Matrigel plug assay and how it can be used for both qualitative and quantitative assessment of angiogenesis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Proteoglycans / Membrane Glycoproteins / Collagen / Laminin / Neovascularization, Physiologic / Endothelial Cells Type of study: Qualitative_research Limits: Animals Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2019 Document type: Article Affiliation country: Greece Country of publication: United States
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Proteoglycans / Membrane Glycoproteins / Collagen / Laminin / Neovascularization, Physiologic / Endothelial Cells Type of study: Qualitative_research Limits: Animals Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2019 Document type: Article Affiliation country: Greece Country of publication: United States