Non-invasive assessment of placental perfusion in vivo using arterial spin labeling (ASL) MRI: A preclinical study in rats.
Placenta
; 77: 39-45, 2019 02.
Article
in En
| MEDLINE
| ID: mdl-30827354
ABSTRACT
INTRODUCTION:
Non-invasive assessment of placental perfusion is of great interest to characterize placental function in clinical practice. This article proposes a strictly non-invasive MRI technique using ASL to quantify placental blood flow in vivo. The aim of this study was to develop a fMRI tool to quantify placental blood flow (PBF) in rat, by using arterial spin labeling (ASL) MRI at 4.7â¯T. MATERIALS ANDMETHODS:
MRI was performed with a dedicated magnet for small animals, in pregnant rats on day 20 of the 22-day gestation period. A Look-Locker flow-sensitive alternating inversion recovery gradient echo sequence was developed as ASL technique (TE 1.55â¯ms; TR 3.5â¯ms, TI 56â¯ms, deltaTI 56â¯ms, FA 20°, Matrix 128â¯×â¯128, 8 segments, 4 Nex). Labeling was performed with global and slice-selective inversions, and T1 map was obtained for each mode of inversion. PBF was then derived from a compartmental model of the variation of T1 between global and slice-selective inversions.RESULTS:
The full protocol was completed and ASL image post-processing was successful in 18 rats. Forty-seven placentas were analyzed, with a mean PBF of 147⯱â¯70â¯ml/min/100â¯g of placenta, consistent with published values of placental perfusion using invasive techniques.CONCLUSION:
ASL MRI is feasible for the quantification of PBF in rats at 4.7â¯T. This technique, which requires no administration of contrast media, could have implications for non-invasive longitudinal and in vivo animal studies and may be useful for the management of human pregnancies.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Placenta
/
Placental Circulation
/
Magnetic Resonance Angiography
Type of study:
Evaluation_studies
/
Guideline
Limits:
Animals
/
Pregnancy
Language:
En
Journal:
Placenta
Year:
2019
Document type:
Article