Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery.
Methods Mol Biol
; 1943: 365-375, 2019.
Article
in En
| MEDLINE
| ID: mdl-30838629
ABSTRACT
Pulmonary siRNA delivery has attracted strong interest and has been reported to successfully mediate target gene knockdown in a number of disease models. However, the nature of the epithelial cells that eventually take up siRNA and the question if other lung cell types may also be transfected has so far been neglected. Therefore, we describe here a flow cytometry-based method using transgenic enhanced green fluorescence protein (EGFP) expressing mice for the differentiation of transfected lung cell populations based on their antigen expression.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transfection
/
RNA, Small Interfering
/
Gene Knockdown Techniques
Limits:
Animals
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2019
Document type:
Article
Affiliation country:
Germany