Propionibacterium acnes-induced immunopathology correlates with health and disease association.
JCI Insight
; 4(5)2019 03 07.
Article
in En
| MEDLINE
| ID: mdl-30843879
ABSTRACT
Genomic studies revealed the existence of health- and acne-associated P. acnes strains and suggested novel approaches for broadening understanding of acne vulgaris. However, clinical association of P. acnes with disease or health has yet to be corroborated experimentally. Current animal models of acne do not closely mimic human disease and have unclear translational value. We have developed a murine model of acne by combining P. acnes inoculation with topical application of a synthetic human sebum. We showed that human sebum promoted persistence of intradermally injected P. acnes with little loss of viability after 1 week and permitted use of more physiologic inoculums. Application of acne-associated P. acnes RT4/5 strains led to development of moderate to severe skin pathology compared with application of health-associated type II P. acnes strains (RT2/6). RT4/5 P. acnes strains uniformly induced higher levels of KC (IL-8), IL-1α, IL-1ß, and IL-6 in vitro and in vivo compared with type II P. acnes strains. Overall, our data provide immunopathologic corroboration of health and disease association of clinical P. acnes strains and inform on a platform to query putative virulence factors uncovered by genomic studies.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Propionibacterium acnes
/
Skin
/
Gram-Positive Bacterial Infections
/
Acne Vulgaris
Type of study:
Prognostic_studies
/
Risk_factors_studies
Limits:
Animals
/
Female
/
Humans
Language:
En
Journal:
JCI Insight
Year:
2019
Document type:
Article
Affiliation country:
United States