Effectiveness of ultra-rapid cryopreservation of sperm from endangered species, examined by morphometric means.
Theriogenology
; 129: 160-167, 2019 Apr 15.
Article
in En
| MEDLINE
| ID: mdl-30852388
ABSTRACT
This study compares the effectiveness of the ultra-rapid and conventional freezing of sperm from captive bovids, giraffids, cervids, ursids, a cercopithecid, a delphinid and a phascolarctid. The relationship between sperm head dimensions and cryosurvival was also examined. Compared to conventional freezing, the ultra-rapid freezing of epididymal sperm from the dama gazelle, giraffe and brown bear returned higher cryoresistance ratios (CR, the ratio, in percentage, between the value of the variable after thawing/value before thawing) for sperm viability and motility. In the remaining species, the conventional freezing of epididymal sperm returned better CR values. The conventional freezing method also returned better CR values for ejaculated samples from all species. The head dimensions of both fresh epididymal and ejaculated sperm differed widely among species for epididymal sperm, dolphin sperm heads were the smallest (7.189⯱â¯0.049⯵m2) and dama gazelle sperm heads the largest (43.746⯱â¯0.291⯵m2), while for ejaculated sperm, giant panda sperm heads were the smallest (15.926⯱â¯0.150⯵m2) and mouflon sperm heads the largest (38.258⯱â¯0.104⯵m2). However, no significant correlations were detected between the CR for motility, viability, membrane functional integrity or acrosome integrity and the sperm head area, either for epididymal or ejaculated sperm. In conclusion, ultra-rapid freezing is especially recommended for the cryopreservation of dama gazelle, giraffe and brown bear epididymal sperm. Sperm head dimensions appear not to be useful predictors of how well sperm might survive freezing.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Semen Preservation
/
Spermatozoa
/
Cryopreservation
/
Endangered Species
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Theriogenology
Year:
2019
Document type:
Article
Affiliation country:
Spain