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Sequencing-Based Transcriptome-Wide Targeted Genotyping for Evolutionary and Ecological Studies.
Zhu, Xuan; Wang, Jing; Lv, Jia; Liu, Pingping; Zhang, Lingling; Jiao, Wenqian; Ma, Cen; Bao, Zhenmin; Wang, Shi.
Affiliation
  • Zhu X; MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China.
  • Wang J; MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China.
  • Lv J; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
  • Liu P; MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China.
  • Zhang L; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
  • Jiao W; MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China.
  • Ma C; MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China.
  • Bao Z; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
  • Wang S; MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China.
Evol Bioinform Online ; 15: 1176934319836074, 2019.
Article in En | MEDLINE | ID: mdl-30886517
ABSTRACT
Transcriptome-wide targeted genotyping is highly attractive for evolutionary and ecological studies but, until recently, accomplishing this goal presented a major technical barrier for the study of non-model organisms. Our group has recently developed a high-throughput targeted genotyping approach (called HD-Marker) based on the high specificity and accuracy of oligo extension-ligation assays that facilitates the design of assays tailored to meet specific genotyping needs. HD-Marker allows for targeted genotyping of over 10 000 genes in a single tube, with strikingly high capture rate (98%-99%) and genotyping accuracy (97%-99%). With the remarkable advantages of cost-effectiveness and flexibility, we envision that HD-Marker has broad application potential in evolutionary and ecological studies.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Evol Bioinform Online Year: 2019 Document type: Article Affiliation country: China
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Evol Bioinform Online Year: 2019 Document type: Article Affiliation country: China