A histopathologic study of the controlling role of T cells on experimental periodontitis in rats.
J Dent Sci
; 13(2): 87-96, 2018 Jun.
Article
in En
| MEDLINE
| ID: mdl-30895102
ABSTRACT
BACKGROUND/PURPOSE:
The onset and progression of periodontitis involve bacterial infection and the immune response. T cells function in the immune response and reportedly induce bone resorption in inflammatory bone loss. However, the exact role of T cells in periodontal destruction remains unclear. Using our experimental model of periodontitis, we aimed to investigate the influence of T cells on periodontal destruction. MATERIALS ANDMETHODS:
Male athymic nude (Nu) and euthymic wild-type (WT) rats were divided into the immunized (I-Nu and I-WT), non-immunized (nI-Nu and nI-WT). The immunized groups were immunized intraperitoneally with lipopolysaccharide (LPS). The non-immunized groups received phosphate-buffered saline (PBS). Nothing was administered to the non-treated groups. LPS was applied to the right palatal gingival sulcus in the immunized and non-immunized groups daily for 20 days. Loss of attachment, numbers of inflammatory cells and osteoclasts, and levels of alveolar bone were investigated histopathologically and histometrically. Osteoclasts were stained with tartrate-resistant acid phosphatase. The numbers of IL-4-positive cells were evaluated immunohistologically.RESULTS:
Loss of attachment, numbers of inflammatory cells, levels of alveolar bone, and the number of osteoclasts were significantly increased in the nI-WT group compared with the nI-Nu group. However, the parameters were significantly increased in the I-Nu group compared with the I-WT group. The number of IL-4-positive cells was greater in the I-WT group than in the I-Nu group.CONCLUSION:
T cells promote inflammation in non-immunized animals; however, they regulate these processes in immunized animals.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
J Dent Sci
Year:
2018
Document type:
Article
Affiliation country:
Japan