Peimine suppresses interleukin1ßinduced inflammation via MAPK downregulation in chondrocytes.
Int J Mol Med
; 43(5): 2241-2251, 2019 May.
Article
in En
| MEDLINE
| ID: mdl-30896805
ABSTRACT
Osteoarthritis (OA) is the most common type of degenerative joint disease and secreted inflammatory molecules serve a pivotal role in it. Peimine has been reported to have antiinflammatory activity. In order to investigate the potential therapeutic role of Peimine in OA, mouse articular chondrocytes were treated with IL1ß and different doses of Peimine in vitro. The data revealed that Peimine not only suppressed IL1ßinduced production of nitric oxide (NO) and prostaglandin E2, but also reduced the protein levels of inducible NO synthase (iNOS) and cyclooxygenase2 (COX2). In addition, Peimine inhibited the IL1ßinduced mRNA expression of matrix metalloproteinase (MMP)1, MMP3, MMP9, MMP13, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)4 and ADAMTS5. Furthermore, Peimine inhibited IL1ßinduced activation of the mitogenactivated protein kinase (MAPK) pathway. The protective effect of Peimine on IL1ßtreated chondrocytes was attenuated following activation of the MAPK pathway, as demonstrated by the increased expression levels of MMP3, MMP13, ADAMTS5, iNOS and COX2 compared with the Peimine group. The in vivo data suggested that Peimine limited the development of OA in the mouse model. In general, the data indicate that Peimine suppresses IL1ßinduced inflammation in mouse chondrocytes by inhibiting the MAPK pathway, suggesting a promising therapeutic role for Peimine in the treatment of OA.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Down-Regulation
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Cevanes
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Chondrocytes
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Mitogen-Activated Protein Kinases
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Inflammation
Limits:
Animals
Language:
En
Journal:
Int J Mol Med
Journal subject:
BIOLOGIA MOLECULAR
/
GENETICA MEDICA
Year:
2019
Document type:
Article