New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies.
J Immunol Methods
; 91(2): 225-9, 1986 Jul 24.
Article
in En
| MEDLINE
| ID: mdl-3090150
ABSTRACT
A new micro-glass-tube leukocyte adherence inhibition (LAI) assay which is appropriate for detecting delayed type hypersensitivity in vitro has been developed for human leukocytes. Enumeration of adherent cells is replaced by a cellular radioimmunoassay determining antibody binding of the monoclonal reagents, OKT4, OKT8 and OKM1, to glass-adherent cells, fixed by glutaraldehyde or formaldehyde. An LAI reactivity to purified protein derivative of tuberculin (PPD) was detectable in donors giving a positive PPD skin test with OKT4 reagent, but not with the other two reagents.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Leukocyte Adherence Inhibition Test
/
Monocytes
/
T-Lymphocytes
/
Immunologic Techniques
/
Antibodies, Monoclonal
Limits:
Humans
Language:
En
Journal:
J Immunol Methods
Year:
1986
Document type:
Article