Effects of carboxyl group on the anticoagulant activity of oxidized carrageenans.
Carbohydr Polym
; 214: 286-293, 2019 Jun 15.
Article
in En
| MEDLINE
| ID: mdl-30925999
ABSTRACT
In this paper, carrageenans having distinct sulfation patterns (κ-, ι-, ι/ν-, θ- and λ-carrageenans), were fully or partially oxidized at C-6 of the ß-d-Galp units using 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) and trichloroisocyanuric acid (TCCA) in bicarbonate buffer. The modified carrageenans were characterized by mono- and bidimensional 1H and 13C NMR spectroscopy. The influence of the sulfate and carboxyl groups onto anticoagulant activity was evaluated using Activated Partial Thromboplastin Time (aPTT) in vitro assay. The results showed a synergic effect of the carboxyl groups on the anticoagulant activity, which was dependent on the regiochemistry of the sulfate groups in the polysaccharide backbone. Sulfate groups at C2 of the ß-d-GalAp units appeared to positively influence the anticoagulant effect in comparison to C4-sulfate samples. Also, the partially oxidized κ-carrageenan derivative (κLO) showed better anticoagulant effect than the fully oxidized carrageenan (κHO).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Uronic Acids
/
Carrageenan
/
Anticoagulants
Language:
En
Journal:
Carbohydr Polym
Year:
2019
Document type:
Article
Affiliation country:
Brazil