Application of qPCR method to hair and cerumen samples for the diagnosis of canine leishmaniosis in Araçatuba, Brazil.
Vet Parasitol Reg Stud Reports
; 15: 100267, 2019 01.
Article
in En
| MEDLINE
| ID: mdl-30929937
ABSTRACT
Visceral leishmaniosis (VL) remains a serious public health problem in Brazil. Dogs are the main hosts of the parasite, developing canine leishmaniosis (CanL), hence the importance of an accurate diagnosis of the animals. Recently, the application of qPCR method to non-invasive samples obtained from dogs with CanL has shown high sensitivity. Thus, we analyzed by qPCR blood, hair (from healthy zones and cutaneous lesions) and cerumen of 16 dogs with confirmed leishmaniosis from Araçatuba, a Brazilian endemic area. Cerumen-qPCR showed the highest sensitivity (87.5%), followed by hair (lesions 78.57%, healthy skin 62.5%), and blood (68.75%). We also analyzed blood, hair and cerumen of 5 healthy dogs from a non-endemic area, obtaining 100% of specificity in all samples. The use of cerumen and hair for qPCR analysis provides high reliability, taking into account the sensitivity and total specificity of the method. The non-invasive sampling procedure without the need of specific conditions of storage and transport support the usefulness of hair and cerumen for the diagnosis of CanL.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cerumen
/
Dog Diseases
/
Real-Time Polymerase Chain Reaction
/
Hair
/
Leishmaniasis, Visceral
Type of study:
Diagnostic_studies
Limits:
Animals
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Vet Parasitol Reg Stud Reports
Year:
2019
Document type:
Article