Preparation of Cells from Embryonic Organs for Single-Cell RNA Sequencing.

Sekiguchi, Rei; Hauser, Belinda

Sekiguchi, Rei; Hauser, Belinda.

Affiliation

Sekiguchi R; Department of Oral and Craniofacial Sciences, School of Dentistry, University of Missouri-Kansas City, Kansas City, Missouri.
Hauser B; Department of Biomedical and Health Informatics, School of Medicine, University of Missouri-Kansas City, Kansas City, Missouri.

Curr Protoc Cell Biol ; 83(1): e86, 2019 06.

Article in En | MEDLINE | ID: mdl-30957983

Subject(s)

Embryo, Mammalian/cytology; Sequence Analysis, RNA/methods; Animals; Cell Separation/methods; Cell Separation/veterinary; Lacrimal Apparatus/cytology; Lacrimal Apparatus/embryology; Mice; Salivary Glands/cytology; Salivary Glands/embryology; Single-Cell Analysis/methods

Key words

cold dissociation; cryophilic protease; embryonic organ; embryonic tissue; scRNA-seq

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sequence Analysis, RNA / Embryo, Mammalian Limits: Animals Language: En Journal: Curr Protoc Cell Biol Year: 2019 Document type: Article Country of publication: United States

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