Experimental Validation of MicroRNA Targets: Luciferase Reporter Assay.

Tomasello, Luisa; Cluts, Landon; Croce, Carlo M

Tomasello, Luisa; Cluts, Landon; Croce, Carlo M.

Affiliation

Tomasello L; Department of Cancer Biology and Medical Genetics, Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA. luisa.tomasello@osumc.edu.
Cluts L; Department of Cancer Biology and Medical Genetics, Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA.
Croce CM; Department of Cancer Biology and Medical Genetics, Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA.

Methods Mol Biol ; 1970: 315-330, 2019.

Article in En | MEDLINE | ID: mdl-30963500

Subject(s)

Computational Biology/methods; Luciferases/metabolism; MicroRNAs/genetics; RNA, Messenger/analysis; Gene Expression Regulation; Genes, Reporter; Humans; Luciferases/genetics; MicroRNAs/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism; Validation Studies as Topic

Key words

Cloning; In vitro techniques; Luciferase assay; MicroRNA target validation

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Messenger / Computational Biology / MicroRNAs / Luciferases Limits: Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2019 Document type: Article Affiliation country: United States Country of publication: United States

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