Sea snake cathelicidin (Hc-cath) exerts a protective effect in mouse models of lung inflammation and infection.
Sci Rep
; 9(1): 6071, 2019 04 15.
Article
in En
| MEDLINE
| ID: mdl-30988402
ABSTRACT
We investigated the anti-inflammatory and antibacterial activities of Hc-cath, a cathelicidin peptide derived from the venom of the sea snake, Hydrophis cyanocyntus, using in vivo models of inflammation and infection. Hc-cath function was evaluated in in vitro, in vivo in the wax moth, Galleria mellonella, and in mouse models of intraperitoneal and respiratory Pseudomonas aeruginosa infection. Hc-Cath downregulated LPS-induced pro-inflammatory responses in macrophages and significantly improved the survival of P. aeruginosa infected G. mellonella over a 5-day period. We also demonstrated, for the first time, that Hc-cath can modulate inflammation in a mouse model of LPS-induced lung inflammation by significantly reducing the release of the pro-inflammatory cytokine and neutrophil chemoattractant, KC, resulting in reduced cellular infiltration into the lungs. Moreover, Hc-cath treatment significantly reduced the bacterial load and inflammation in mouse models of P. aeruginosa intraperitoneal and respiratory infection. The effect of Hc-cath in our studies highlights the potential to develop this peptide as a candidate for therapeutic development.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pneumonia
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Pseudomonas Infections
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Biological Products
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Antimicrobial Cationic Peptides
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Hydrophiidae
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Anti-Infective Agents
Limits:
Animals
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Female
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Humans
Language:
En
Journal:
Sci Rep
Year:
2019
Document type:
Article
Affiliation country:
United kingdom