Characterization and engineering of the Lrp/AsnC family regulator SACE_5717 for erythromycin overproduction in Saccharopolyspora erythraea.
J Ind Microbiol Biotechnol
; 46(7): 1013-1024, 2019 Jul.
Article
in En
| MEDLINE
| ID: mdl-31016583
In this work, we found that the Lrp/AsnC family protein SACE_5717 negatively regulated erythromycin biosynthesis in S. erythraea. Disruption of SACE_5717 led to a 27% improvement in the yield of erythromycin in S. erythraea A226. SACE_5717 directly repressed its own gene expression, as well as that of the adjacent gene SACE_5716 by binding to the target sequence 5'-GAACGTTCGCCGTCACGCC-3'. The predicted LysE superfamily protein SACE_5716 directly influenced the export of lysine, histidine, threonine and glycine in S. erythraea. Arginine, tyrosine and tryptophan were characterized as the effectors of SACE_5717 by weakening the binding affinity of SACE_5717. In the industrial S. erythraea WB strain, deletion of SACE_5717 (WBΔSACE_5717) increased erythromycin yield by 20%, and by 36% when SACE_5716 was overexpressed in WBΔSACE_5717 (WBΔSACE_5717/5716). In large-scale 5-L fermentation experiment, erythromycin yield in the engineered strain WBΔSACE_5717/5716 reached 4686 mg/L, a 41% enhancement over 3323 mg/L of the parent WB strain.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Erythromycin
/
Saccharopolyspora
Language:
En
Journal:
J Ind Microbiol Biotechnol
Journal subject:
BIOTECNOLOGIA
/
MICROBIOLOGIA
Year:
2019
Document type:
Article
Affiliation country:
China
Country of publication:
Germany