L-threo-3,4-dihydroxyphenylserine (DOPS) aldolase: a new enzyme cleaving DOPS into protocatechualdehyde and glycine.
Biochem Biophys Res Commun
; 143(2): 482-8, 1987 Mar 13.
Article
in En
| MEDLINE
| ID: mdl-3105531
ABSTRACT
An enzyme which cleaves L-threo-3,4-dihydroxyphenylserine into protocatechualdehyde and glycine was demonstrated in extracts of human brains. Equimolar production of protocachualdehyde and glycine was quantitatively confirmed using high-performance liquid chromatography. In subcellular fractions of the brain, the highest enzyme activity was found in cytosol and soluble fraction. L-threo-DOPS proved to be the best substrate for this enzyme. The L-erythroisomer was less active and D-threo- and D-erythro-isomers were essentially inactive. The enzyme activity has an optimal pH around 7.4, and requires pyridoxal phosphate for maximal activity.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Serine
/
Cerebral Cortex
/
Droxidopa
/
Aldehyde-Lyases
Limits:
Humans
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
1987
Document type:
Article