Adenylate cyclase of bovine adrenal cortex plasma membranes. Divergence between corticotropin and fluoride combined effects with forskolin.
FEBS Lett
; 216(1): 107-12, 1987 May 25.
Article
in En
| MEDLINE
| ID: mdl-3108032
ABSTRACT
The diterpene forskolin maximally stimulated bovine adrenal cortex adenylate cyclase activity 9-fold with a concentration producing half-maximum effect (ED50) of about 4 microM. The effects of forskolin and the fully active corticotropin fragment ACTH (I 24) were additive over nearly the whole range of concentration of both effectors, indicating separate and independent mechanisms of action. By contrast, 10 mM NaF blocked forskolin action in the nanomolar range of the diterpene concentration, while it allowed a partial stimulation by forskolin in the micromolar range. NaF thus reveals a heterogeneity of forskolin action in the adrenal cortex plasma membranes. Moreover, our data suggest that ACTH and NaF activation effects, both mediated by the stimulatory regulatory protein Gs, proceed through different mechanisms.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Adenylyl Cyclases
/
Adrenal Cortex
Limits:
Animals
Language:
En
Journal:
FEBS Lett
Year:
1987
Document type:
Article