High-level secretive expression of a novel achieved Talaromyces cellulolyticus endo-polygalacturonase in Pichia pastoris by improving gene dosage for hydrolysis of natural pectin.

Peng, Xiao-Bo; Chen, Guang-Jun; Han, Zhen-Gang; Yang, Jiang-Ke

Peng, Xiao-Bo; Chen, Guang-Jun; Han, Zhen-Gang; Yang, Jiang-Ke.

Affiliation

Peng XB; College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, 430023, China.
Chen GJ; College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, 430023, China.
Han ZG; College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, 430023, China.
Yang JK; College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, 430023, China. jiangke.yang@gmail.com.

World J Microbiol Biotechnol ; 35(6): 84, 2019 May 27.

Article in En | MEDLINE | ID: mdl-31134444

Subject(s)

Gene Dosage; Pectins/metabolism; Pichia/genetics; Polygalacturonase/biosynthesis; Polygalacturonase/genetics; Talaromyces/enzymology; Talaromyces/genetics; Bioreactors; Cloning, Molecular; Gene Expression Regulation, Fungal; Hydrolysis; Pichia/metabolism; Real-Time Polymerase Chain Reaction/methods; Recombinant Proteins/genetics; Temperature; Time Factors

Key words

Gene dosage; Parameters optimization; Pectinase; Real time quantitative PCR; Thermal stable

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pichia / Polygalacturonase / Pectins / Gene Dosage / Talaromyces Language: En Journal: World J Microbiol Biotechnol Year: 2019 Document type: Article Affiliation country: China

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