Expressions of TGF-ß1 and Eâ
¢A-FN after Rat Skeletal Muscle Contusion and Wound Age Estimation.
Fa Yi Xue Za Zhi
; 35(2): 154-159, 2019 Apr.
Article
in En, Zh
| MEDLINE
| ID: mdl-31135108
ABSTRACT
ABSTRACT Objective To study the expressions of transforming growth factor-ß1 ï¼TGF-ß1ï¼ and Eâ
¢A-fibronectin ï¼Eâ
¢A-FNï¼ at different time points of antemortem injury, antemortem injury postmortem expression and postmortem injury and to explore their application value in wound age estimation. Methods A model of rat skeletal muscle contusion was established. The rats were randomly divided into normal control group ï¼n=5ï¼, antemortem contusion group ï¼n=40ï¼, antemortem contusion postmortem expression group ï¼n=110ï¼ and postmortem injury group ï¼n=25ï¼. The expressions of TGF-ß1 and Eâ
¢A-FN after rat skeletal muscles antemortem contusion were detected with immunohistochemical staining. Expression changes of TGF-ß1 and Eâ
¢A-FN mRNA in each group were analyzed with real-time fluorescence quantitative PCR. Results Immunohistochemical staining results showed that a large number of polymorphonuclear leukocyte, mononuclear cells and fibroblastic cells showed a strong expression of TGF-ß1 in wounded zones 12 h-14 d after antemortem contusion. Eâ
¢A-FN was mainly distributed in the extracellular matrix, 3 to 7 d post-traumatic. Real-time fluorescence quantitative PCR results showed that TGF-ß1 and Eâ
¢A-FN mRNA in antemortem injury group reached the peak at 3 and 5 d post-traumatic respectively. The expressions of TGF-ß1 and Eâ
¢A-FN mRNA in antemortem contusion postmortem expression group peaked at 6 h and 12 h postmortem. The expression of TGF-ß1 and Eâ
¢A-FN mRNA in postmortem injury group 0.5-12 h postmortem was significantly lower than those of the normal control group and the antemortem contusion group. Conclusion TGF-ß1 and Eâ
¢A-FN might become a reference index for skeletal muscle wound age estimation.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Fibronectins
/
Muscle, Skeletal
/
Contusions
/
Transforming Growth Factor beta1
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
/
Zh
Journal:
Fa Yi Xue Za Zhi
Journal subject:
JURISPRUDENCIA
Year:
2019
Document type:
Article
Affiliation country:
China