Methanol extract of Cola nitida ameliorates inflammation and nociception in experimental animals.
Neurobiol Pain
; 5: 100027, 2019.
Article
in En
| MEDLINE
| ID: mdl-31194116
ABSTRACT
Methanol extract of Cola nitida (MECN) was evaluated for its anti-inflammatory and analgesic activities using rats and mice. Inflammatory activity of MECN was assessed by carrageenan-induced paw oedema while analgesic activity was evaluated by acetic acid -induced writhing and formalin paw lick test. Histological analyses of the paws were also carried out. There was evaluation of the mechanism(s) of action of MECN using naloxone, a blocker of opioid receptors; atropine, blocker of muscarinic receptors; and propranolol, blocker of beta adrenergic receptors. Findings from the study revealed that MECN has both anti-inflammatory and analgesic properties. These properties were found to be dose dependent with 200â¯mg/kg of MECN discovered to be the most potent dose. 200â¯mg/kg was able to cause statistically significant reduction in paw size (pâ¯<â¯0.001) when compared with the carrageenan group. Histological analysis revealed that rats treated with 200â¯mg/kg of MECN showed no inflammatory cells in the left paw compared to other groups treated with carrageenan. In the formalin test, the number of paw licking was significantly reduced by MECN at 50â¯mg/kg, 100â¯mg/kg and 200â¯mg/kg in both neurogenic and inflammatory pain responses (pâ¯<â¯0.001) even as 200â¯mg/kg showed the highest percentage inhibition of 98.17% while 100â¯mg/kg of aspirin showed percentage inhibition of 93.66%. In acetic acid-induced writhing test, 50â¯mg/kg, 100â¯mg/kg and 200â¯mg/kg of MECN produced significant inhibition of writhes when compared with control as highest inhibition is observed in mice that received 200â¯mg/kg which is similar to aspirin. Administration of propranolol and naloxone was unable to reverse the analgesic function of MECN. However, atropine administration blocked the analgesic function of MECN. This shows that MECN exhibits its analgesic property through cholinergic pathway and not opioid and adrenergic pathways. Phytochemical screening revealed that MECN contains flavonoids, steroids, saponins, tannins, anthraquinines, terpenoids, and alkanoids. These phytochemical contents may thus be responsible for its analgesic and anti-inflammatory properties.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Neurobiol Pain
Year:
2019
Document type:
Article
Affiliation country:
Nigeria