Combination of SCX Fractionation and Charge-Reversal Derivatization Facilitates the Identification of Nontryptic Peptides in C-Terminomics.

Kaleja, Patrick; Helbig, Andreas O; Tholey, Andreas

Kaleja, Patrick; Helbig, Andreas O; Tholey, Andreas.

Affiliation

Kaleja P; Systematic Proteome Research & Bioanalytics, Institute for Experimental Medicine , Christian-Albrechts-Universität zu Kiel , 24105 Kiel , Germany.
Helbig AO; Systematic Proteome Research & Bioanalytics, Institute for Experimental Medicine , Christian-Albrechts-Universität zu Kiel , 24105 Kiel , Germany.
Tholey A; Systematic Proteome Research & Bioanalytics, Institute for Experimental Medicine , Christian-Albrechts-Universität zu Kiel , 24105 Kiel , Germany.

J Proteome Res ; 18(7): 2954-2964, 2019 07 05.

Article in En | MEDLINE | ID: mdl-31195796

Subject(s)

Peptides/analysis; Proteolysis; Proteomics/methods; Carboxypeptidases; Chromatography, Liquid; Isotope Labeling; Proteome/analysis; Saccharomyces cerevisiae/chemistry; Tandem Mass Spectrometry/methods

Key words

O-labeling; PICS; TAILS; cyanogen bromide; fragmentation; proteolysis; ragged peptides; solid-phase derivatization; trypsin specificity

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptides / Proteomics / Proteolysis Type of study: Diagnostic_studies / Prognostic_studies Language: En Journal: J Proteome Res Journal subject: BIOQUIMICA Year: 2019 Document type: Article Affiliation country: Germany Country of publication: United States

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