Chemical Modification of 1-Aminocyclopropane Carboxylic Acid (ACC) Oxidase: Cysteine Mutational Analysis, Characterization, and Bioconjugation with a Nitroxide Spin Label.

Tachon, Sybille; Fournier, Eugénie; Decroos, Christophe; Mansuelle, Pascal; Etienne, Emilien; Maresca, Marc; Martinho, Marlène; Belle, Valérie; Tron, Thierry; Simaan, Ariane Jalila

Tachon, Sybille; Fournier, Eugénie; Decroos, Christophe; Mansuelle, Pascal; Etienne, Emilien; Maresca, Marc; Martinho, Marlène; Belle, Valérie; Tron, Thierry; Simaan, Ariane Jalila.

Affiliation

Tachon S; CNRS, Aix Marseille Univ, Centrale Marseille, iSm2, Marseille, France.
Fournier E; CNRS, Aix Marseille Univ, Centrale Marseille, iSm2, Marseille, France.
Decroos C; BIP, CNRS, Aix Marseille Univ, Marseille, France.
Mansuelle P; CNRS, Aix Marseille Univ, Centrale Marseille, iSm2, Marseille, France.
Etienne E; Plate-forme Protéomique, Marseille Protéomique (MaP), IBiSA Labeled, FR3479 Institut de Microbiologie de la Méditerranée, CNRS, Aix Marseille Univ, Marseille, France.
Maresca M; BIP, CNRS, Aix Marseille Univ, Marseille, France.
Martinho M; CNRS, Aix Marseille Univ, Centrale Marseille, iSm2, Marseille, France.
Belle V; BIP, CNRS, Aix Marseille Univ, Marseille, France.
Tron T; BIP, CNRS, Aix Marseille Univ, Marseille, France. belle@imm.cnrs.fr.
Simaan AJ; CNRS, Aix Marseille Univ, Centrale Marseille, iSm2, Marseille, France.

Mol Biotechnol ; 61(9): 650-662, 2019 Sep.

Article in En | MEDLINE | ID: mdl-31201604

Subject(s)

Amino Acid Oxidoreductases/chemistry; Amino Acids, Cyclic/chemistry; Cysteine/chemistry; Ethylenes/chemistry; Nitrogen Oxides/chemistry; Plant Proteins/chemistry; Solanum lycopersicum/enzymology; Amino Acid Oxidoreductases/genetics; Amino Acid Oxidoreductases/metabolism; Amino Acid Substitution; Amino Acids, Cyclic/metabolism; Binding Sites; Cloning, Molecular; Cysteine/metabolism; Electron Spin Resonance Spectroscopy; Escherichia coli/genetics; Escherichia coli/metabolism; Ethylenes/biosynthesis; Gene Expression; Genetic Vectors/chemistry; Genetic Vectors/metabolism; Kinetics; Solanum lycopersicum/chemistry; Models, Molecular; Mutagenesis, Site-Directed; Nitrogen Oxides/metabolism; Plant Proteins/genetics; Plant Proteins/metabolism; Protein Binding; Protein Conformation, alpha-Helical; Protein Conformation, beta-Strand; Protein Interaction Domains and Motifs; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Spin Labels; Substrate Specificity

Key words

ACC oxidase; Cysteine; EPR; Ethylene; Iron; Mutagenesis; Nitroxide; Protein chemical modification

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Proteins / Solanum lycopersicum / Cysteine / Ethylenes / Amino Acid Oxidoreductases / Amino Acids, Cyclic / Nitrogen Oxides Language: En Journal: Mol Biotechnol Journal subject: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Year: 2019 Document type: Article Affiliation country: France Country of publication: Switzerland

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