Methods for Optical Skin Clearing in Molecular Optical Imaging in Dermatology.
Biochemistry (Mosc)
; 84(Suppl 1): S144-S158, 2019 Jan.
Article
in En
| MEDLINE
| ID: mdl-31213200
ABSTRACT
This short review describes recent progress in using optical clearing (OC) technique in skin studies. Optical clearing is an efficient tool for enhancing the probing depth and data quality in multiphoton microscopy and Raman spectroscopy. Here, we discuss the main mechanisms of OC, its safety, advantages, and limitations. The data on the OC effect on the skin water content are presented. It was demonstrated that 70% glycerol and 100% OmnipaqueTM 300 reduce the water content in the skin. Both OC agents (OCAs) significantly affect the strongly bound and weakly bound water. However, OmnipaqueTM 300 causes considerably less skin dehydration than glycerol. In addition, the results of examination of the OC effect on autofluorescence in two-photon excitation and background fluorescence in Raman scattering at different skin depths are presented. It is shown that OmnipaqueTM 300 is a promising OCA due to its ability to reduce background fluorescence in the upper skin layers. The possibility of multimodal imaging combining optical methods and OC technique is discussed.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Skin
/
Spectrum Analysis, Raman
/
Microscopy, Fluorescence, Multiphoton
Limits:
Humans
Language:
En
Journal:
Biochemistry (Mosc)
Year:
2019
Document type:
Article
Affiliation country:
Finland