Protective effect of hypoxylonol C and 4,5,4',5'-tetrahydroxy-1,1'-binaphthyl isolated from Annulohypoxylon annulatum against streptozotocin-induced damage in INS-1 cells.
Bioorg Chem
; 90: 103053, 2019 09.
Article
in En
| MEDLINE
| ID: mdl-31220671
ABSTRACT
We evaluated the protective effects of hypoxylonol C and 4,5,4',5'-tetrahydroxy-1,1'-binaphthyl (BNT) isolated from Annulohypoxylon annulatum on pancreatic ß-cell apoptosis, using the ß-cell toxin streptozotocin (STZ). Hypoxylonol C and BNT restored the STZ-induced decrease in INS-1 cell viability in a dose-dependent manner. In addition, treatment of INS-1 cells with 50⯵M STZ resulted in an increase in apoptotic cell death, which was observed as annexin V fluorescence intensity. Apoptotic cell death was decreased by co-treatment with 100⯵M hypoxylonol C and 100⯵M BNT. Similarly, STZ caused a marked increase in the expression of cleaved caspase-8, caspase-3, Bax, and poly (ADP-ribose) polymerase (PARP), as well as a decrease in the expression of B-cell lymphoma 2 (Bcl-2), which was reversed by co-treatment with 100⯵M hypoxylonol C and 100⯵M BNT. These findings suggest that hypoxylonol C and BNT play an important role in protecting pancreatic ß-cells against apoptotic damage.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Streptozocin
/
Protective Agents
/
Fluorenes
/
Naphthols
Limits:
Animals
Language:
En
Journal:
Bioorg Chem
Year:
2019
Document type:
Article