Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation.
J Vis Exp
; (148)2019 06 07.
Article
in En
| MEDLINE
| ID: mdl-31233018
ABSTRACT
We report that mRNA electroporation permits fluorescent proteins to label cells in living quail embryos more quickly and broadly than DNA electroporation. The high transfection efficiency permits at least 4 distinct mRNAs to be co-transfected with ~87% efficiency. Most of the electroporated mRNAs are degraded during the first 2 h post-electroporation, permitting time-sensitive experiments to be carried out in the developing embryo. Finally, we describe how to dynamically image live embryos electroporated with mRNAs that encode various subcellular targeted fluorescent proteins.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Quail
/
Electroporation
/
Embryo, Nonmammalian
/
Luminescent Proteins
Limits:
Animals
Language:
En
Journal:
J Vis Exp
Year:
2019
Document type:
Article